Device

Part:BBa_K3226020:Design

Designed by: Yuma matsumoto   Group: iGEM19_Botchan_Lab_Tokyo   (2019-10-16)


Add the his tag to BBa_K602008.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 3396
    Illegal suffix found in sequence at 1348
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3396
    Illegal SpeI site found at 1349
    Illegal PstI site found at 1363
    Illegal NotI site found at 1356
    Illegal NotI site found at 3402
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3396
    Illegal BglII site found at 468
    Illegal XhoI site found at 2380
    Illegal XhoI site found at 3272
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 3396
    Illegal suffix found in sequence at 1349
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 3396
    Illegal XbaI site found at 3411
    Illegal SpeI site found at 1349
    Illegal PstI site found at 1363
    Illegal NgoMIV site found at 1142
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We made this parts by using in-fusion cloning. The homologous sequence is needed between insert with vector to do in-fusion cloning. In this way, we took care not to make a mistake in the operation part of the infusion cloning that there is not in the restriction enzyme operation. Please refer to the dedicated page for detailed protocol.

Source

Deinococcus Radiodurans

References

・Tomoyasu Aizawa., (2008)., Ni-NTA affinity column., Protein Science Society archive#019., Protein Science Society of Japan.

・In-Fusion® HD Cloning Kit User Manual., Takara Bio USA, Inc., p.3-4.